library(tidyverse)
library(tidylog)
library(broom)
library(RColorBrewer)
library(viridis)
library(minpack.lm)
library(patchwork)
library(ggtext)
library(brms)
library(modelr)
library(tidybayes)
library(ggridges)
library(performance)
# devtools::install_github("seananderson/ggsidekick") ## not on CRAN
library(ggsidekick); theme_set(theme_sleek())
# Load functions
home <- here::here()
fxn <- list.files(paste0(home, "/R/functions"))
invisible(sapply(FUN = source, paste0(home, "/R/functions/", fxn)))Fit VBGE models to back-calculated length-at-age
Load libraries
Load cache
# qwraps2::lazyload_cache_dir(path = paste0(home, "/R/analyze-data/02-fit-vbge_cache/html"))Read and trim data
d <- #read_csv(paste0(home, "/data/for-analysis/dat.csv")) %>%
read_csv("https://raw.githubusercontent.com/maxlindmark/perch-growth/master/data/for-analysis/dat.csv") %>%
filter(age_ring == "Y", # use only length-at-age by filtering on age_ring
!area %in% c("VN", "TH")) Rows: 338460 Columns: 12
── Column specification ────────────────────────────────────────────────────────
Delimiter: ","
chr (6): age_ring, area, gear, ID, sex, keep
dbl (6): length_mm, age_bc, age_catch, catch_year, cohort, final_length
ℹ Use `spec()` to retrieve the full column specification for this data.
ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message.
filter: removed 43,886 rows (13%), 294,574 rows remaining# Minimum number of observations per area and cohort
d$area_cohort <- as.factor(paste(d$area, d$cohort))
d <- d %>%
group_by(area_cohort) %>%
filter(n() > 100) %>%
ungroup()group_by: one grouping variable (area_cohort)
filter (grouped): removed 2,637 rows (1%), 291,937 rows remaining
ungroup: no grouping variables# Minimum number of observations per area, cohort, and age
d$area_cohort_age <- as.factor(paste(d$area, d$cohort, d$age_bc))
d <- d %>%
group_by(area_cohort_age) %>%
filter(n() > 10) %>%
ungroup()group_by: one grouping variable (area_cohort_age)
filter (grouped): removed 3,521 rows (1%), 288,416 rows remaining
ungroup: no grouping variables# Minimum number of cohorts in a given area
cnt <- d %>%
group_by(area) %>%
summarise(n = n_distinct(cohort)) %>%
filter(n >= 10)group_by: one grouping variable (area)
summarise: now 10 rows and 2 columns, ungrouped
filter: no rows removedd <- d[d$area %in% cnt$area, ]
# Plot cleaned data
ggplot(d, aes(age_bc, length_mm)) +
geom_jitter(size = 0.1, height = 0, alpha = 0.1) +
scale_x_continuous(breaks = seq(20)) +
theme(axis.text.x = element_text(angle = 0)) +
theme(axis.text = element_text(size = 12), axis.title = element_text(size = 15)) +
labs(x = "Age", y = "Length (mm)") +
guides(color = "none") +
facet_wrap(~area, scale = "free_x")
# Longitude and latitude attributes for each area
area <- c("BS", "BT", "FB", "FM", "HO", "JM", "MU", "RA", "SI_EK", "SI_HA", "TH")
nareas <- length(area)
lat <- c(60, 60.4, 60.3, 60.5, 63.7, 58, 59, 65.9, 57.3, 57.4, 56.1)
lon <- c(21.5, 18.1, 19.5, 18, 20.9, 16.8, 18.1, 22.3, 16.6, 16.7, 15.9)
area_attr <- data.frame(cbind(area = area, lat = lat, lon = lon)) %>%
mutate_at(c("lat", "lon"), as.numeric)mutate_at: converted 'lat' from character to double (0 new NA)
converted 'lon' from character to double (0 new NA)Fit VBGE models
# Get individual growth parameters (functions: VBGF/Gompertz,nls_out,fit_nls)
IVBG <- d %>%
group_by(ID) %>%
summarize(nls_out(fit_nls(length_mm, age_bc, min_nage = 5, model = "VBGF"))) %>%
ungroup()group_by: one grouping variable (ID)summarize: now 75,245 rows and 5 columns, ungroupedungroup: no grouping variables# Test what's going on ...
# expand_grid(k = c(0.1, 0.2),
# linf = c(300, 400)) %>%
# crossing(age_bc = 1:100) %>%
# mutate(length_mm_pred = linf*(1-exp(-k*age_bc)),
# group = paste0("k = ", k, " and Linf = ", linf)) %>%
# ggplot(aes(age_bc, length_mm_pred, color = group)) +
# geom_line()IVBG <- IVBG %>% drop_na(k) # The NAs are because the model didn't converge or because they were below the threshold agedrop_na: removed 51,638 rows (69%), 23,607 rows remainingIVBG <- IVBG %>%
mutate(k_lwr = k - 1.96*k_se,
k_upr = k + 1.96*k_se,
linf_lwr = linf - 1.96*linf_se,
linf_upr = linf + 1.96*linf_se,
A = k*linf*0.65,
row_id = row_number())mutate: new variable 'k_lwr' (double) with 23,605 unique values and 0% NA new variable 'k_upr' (double) with 23,605 unique values and 0% NA new variable 'linf_lwr' (double) with 23,605 unique values and 0% NA new variable 'linf_upr' (double) with 23,605 unique values and 0% NA new variable 'A' (double) with 23,605 unique values and 0% NA new variable 'row_id' (integer) with 23,607 unique values and 0% NA# Add back cohort and area variables
IVBG <- IVBG %>%
left_join(d %>% select(ID, area_cohort)) %>%
separate(area_cohort, into = c("area", "cohort"), remove = TRUE, sep = " ") %>%
mutate(cohort = as.numeric(cohort))select: dropped 12 variables (length_mm, age_bc, age_catch, age_ring, area, …)Joining with `by = join_by(ID)`
left_join: added one column (area_cohort)
> rows only in x 0
> rows only in y (146,384)
> matched rows 142,032 (includes duplicates)
> =========
> rows total 142,032
mutate: converted 'cohort' from character to double (0 new NA)# Compare how the means and quantiles differ depending on this filtering
IVBG_filter <- IVBG %>%
filter(k_se < quantile(k_se, probs = 0.95))filter: removed 7,102 rows (5%), 134,930 rows remaining# Summarize growth coefficients by cohort and area
VBG <- IVBG %>%
filter(k_se < k) %>% # new!
group_by(cohort, area) %>%
summarize(k = quantile(k, prob = 0.5, na.rm = T),
A = quantile(A, prob = 0.5, na.rm = T),
linf = quantile(linf, prob = 0.5, na.rm = T),
k_lwr = quantile(k, prob = 0.05, na.rm = T),
k_upr = quantile(k, prob = 0.95, na.rm = T)) %>%
ungroup()filter: removed 4,201 rows (3%), 137,831 rows remaining
group_by: 2 grouping variables (cohort, area)
summarize: now 306 rows and 7 columns, one group variable remaining (cohort)
ungroup: no grouping variablesCalculate sample size
sample_size <- IVBG %>%
group_by(area) %>%
summarise(n_cohort = length(unique(cohort)),
min_cohort = min(cohort),
max_cohort = max(cohort),
n_individuals = length(unique(ID)),
n_data_points = n())group_by: one grouping variable (area)summarise: now 10 rows and 6 columns, ungroupedsample_size# A tibble: 10 × 6
area n_cohort min_cohort max_cohort n_individuals n_data_points
<chr> <int> <dbl> <dbl> <int> <int>
1 BS 17 1985 2001 1334 7688
2 BT 22 1972 1995 961 5371
3 FB 47 1969 2015 6040 37381
4 FM 37 1963 1999 5055 32632
5 HO 29 1982 2015 1151 6215
6 JM 60 1953 2014 4866 28739
7 MU 18 1981 1999 1105 6671
8 RA 14 1985 2003 571 3115
9 SI_EK 24 1968 2011 659 3577
10 SI_HA 38 1967 2013 1865 10643sample_size %>%
ungroup() %>%
summarise(sum_ind = sum(n_individuals), sum_n = sum(n_data_points))ungroup: no grouping variablessummarise: now one row and 2 columns, ungrouped# A tibble: 1 × 2
sum_ind sum_n
<int> <int>
1 23607 142032write_csv(sample_size, paste0(home, "/output/sample_size.csv"))Add GAM-predicted temperature to growth models
pred_temp <- read_csv(paste0(home, "/output/gam_predicted_temps.csv")) %>%
rename(cohort = year)Rows: 380 Columns: 4
── Column specification ────────────────────────────────────────────────────────
Delimiter: ","
chr (2): area, model
dbl (2): year, temp
ℹ Use `spec()` to retrieve the full column specification for this data.
ℹ Specify the column types or set `show_col_types = FALSE` to quiet this message.
rename: renamed one variable (cohort)VBG <- VBG %>%
left_join(pred_temp, by = c("area", "cohort"))left_join: added 2 columns (temp, model)
> rows only in x 0
> rows only in y ( 74)
> matched rows 306
> =====
> rows total 306# Save data for map-plot
cohort_sample_size <- IVBG %>%
group_by(area, cohort) %>%
summarise(n = n()) # individuals, not samples!group_by: 2 grouping variables (area, cohort)
summarise: now 306 rows and 3 columns, one group variable remaining (area)VBG <- left_join(VBG, cohort_sample_size, by = c("cohort", "area"))left_join: added one column (n)
> rows only in x 0
> rows only in y ( 0)
> matched rows 306
> =====
> rows total 306write_csv(VBG, paste0(home, "/output/vbg.csv"))
# Calculate the plotting order (also used for map plot)
order <- VBG %>%
ungroup() %>%
group_by(area) %>%
summarise(mean_temp = mean(temp)) %>%
arrange(desc(mean_temp))ungroup: no grouping variables
group_by: one grouping variable (area)
summarise: now 10 rows and 2 columns, ungroupedorder# A tibble: 10 × 2
area mean_temp
<chr> <dbl>
1 SI_HA 13.6
2 BT 13.1
3 FM 8.90
4 JM 8.77
5 BS 8.44
6 FB 8.16
7 SI_EK 8.03
8 MU 6.85
9 HO 6.58
10 RA 6.50write_csv(order, paste0(home, "/output/ranked_temps.csv"))
nareas <- length(unique(order$area)) + 2 # to skip the brightest colors that are hard to read
colors <- colorRampPalette(brewer.pal(name = "RdYlBu", n = 10))(nareas)[-c(6,7)]Plot VBGE fits
# Sample 30 IDs per area and plot their data and VBGE fits
set.seed(4)
ids <- IVBG %>% distinct(ID, .keep_all = TRUE) %>% group_by(area) %>% slice_sample(n = 30)distinct: removed 118,425 rows (83%), 23,607 rows remaininggroup_by: one grouping variable (area)slice_sample (grouped): removed 23,307 rows (99%), 300 rows remainingIVBG2 <- IVBG %>%
filter(ID %in% ids$ID) %>%
distinct(ID, .keep_all = TRUE) %>%
select(ID, k, linf)filter: removed 140,311 rows (99%), 1,721 rows remainingdistinct: removed 1,421 rows (83%), 300 rows remainingselect: dropped 10 variables (k_se, linf_se, k_lwr, k_upr, linf_lwr, …)d2 <- d %>%
ungroup() %>%
filter(ID %in% ids$ID) %>%
left_join(IVBG2, by = "ID") %>%
mutate(length_mm_pred = linf*(1-exp(-k*age_bc)))ungroup: no grouping variablesfilter: removed 286,695 rows (99%), 1,721 rows remainingleft_join: added 2 columns (k, linf) > rows only in x 0 > rows only in y ( 0) > matched rows 1,721 > ======= > rows total 1,721mutate: new variable 'length_mm_pred' (double) with 1,714 unique values and 0% NAfits_ind <- ggplot(d2, aes(age_bc, length_mm, group = ID, color = ID)) +
geom_jitter(width = 0.3, height = 0, alpha = 0.5, size = 0.4) +
geom_line(aes(age_bc, length_mm_pred, group = ID, color = ID),
inherit.aes = FALSE, alpha = 0.8, linewidth = 0.3) +
guides(color = "none") +
scale_color_viridis(discrete = TRUE, name = "Site", option = "cividis") +
scale_x_continuous(breaks = seq(1, 20, by = 2)) +
labs(x = "Age (years)", y = "Length (mm)") +
facet_wrap(~factor(area, levels = (arrange(area_attr, desc(lat)))$area), ncol = 5)
A <- IVBG %>%
ggplot(aes(factor(area, order$area), A, fill = factor(area, order$area))) +
coord_cartesian(ylim = c(22, 90)) +
geom_violin(alpha = 0.8, color = NA) +
geom_boxplot(outlier.color = NA, width = 0.2, alpha = 0.9, fill = NA, size = 0.4) +
scale_fill_manual(values = colors, name = "Site") +
scale_color_manual(values = colors, name = "Site") +
guides(fill = "none", color = "none") +
labs(x = "Site", y = "Growth coefficient (*A*)") +
theme(axis.title.y = ggtext::element_markdown())
fits_ind / A + plot_annotation(tag_levels = "A") #+ plot_layout(heights = c(1, 1.8))
ggsave(paste0(home, "/figures/vb_pars_fits.pdf" ), width = 17, height = 18, units = "cm")
# Supp plot for K and Linf
k <- IVBG %>%
ggplot(aes(factor(area, order$area), k, fill = factor(area, order$area))) +
coord_cartesian(ylim = c(0, 0.7)) +
geom_violin(alpha = 0.8, color = NA) +
geom_boxplot(outlier.color = NA, width = 0.2, alpha = 0.9, fill = NA, size = 0.4) +
scale_fill_manual(values = colors, name = "Site") +
guides(fill = "none", color = "none") +
labs(x = "Site", y = expression(italic(k)))
linf <- IVBG %>%
filter(linf < 2300) %>%
filter(linf > 130) %>%
ggplot(aes(factor(area, order$area), linf, fill = factor(area, order$area))) +
geom_violin(alpha = 0.8, color = NA) +
geom_boxplot(outlier.color = NA, width = 0.1, alpha = 0.9, fill = NA, size = 0.4) +
coord_cartesian(ylim = c(0, 2000)) +
scale_fill_manual(values = colors, name = "Site") +
guides(fill = "none", color = "none") +
labs(x = "", y = expression(paste(italic(L[infinity]), " [mm]")))filter: removed 1,227 rows (1%), 140,805 rows remainingfilter: no rows removedk / linf
ggsave(paste0(home, "/figures/supp/vb_k_linf.pdf" ), width = 17, height = 18, units = "cm")Fit Models
Overall, \(A\) looks very linearly related to temperature in most cases, even when I add gams on top
dat <- VBG %>%
mutate(temp_sc = (temp - mean(temp)) / sd(temp),
temp_sq_sc = temp_sc * temp_sc,
area_f = as.factor(area))mutate: new variable 'temp_sc' (double) with 294 unique values and 0% NA new variable 'temp_sq_sc' (double) with 294 unique values and 0% NA new variable 'area_f' (factor) with 10 unique values and 0% NA# Non-scaled x-axis
scatter <- ggplot(dat, aes(temp, A, color = factor(area, order$area)), size = 0.6) +
geom_point(size = 0.9) +
scale_color_manual(values = colors, name = "Site") +
scale_fill_manual(values = colors, name = "Site")
scatter +
geom_smooth(method = "gam", formula = y~s(x, k=3), se = FALSE)
Now we’ll compare a bunch of models different temperature shapes and site-effects
# Quadratic effect of temperature
m1 <- brm(A ~ area_f + temp_sc + temp_sq_sc,
data = dat,
cores = 4,
chains = 4,
iter = 4000,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE),
seed = 99
)
# Interaction between area and temperature
m2 <- brm(A ~ area_f * temp_sc,
data = dat,
cores = 4,
chains = 4,
iter = 4000,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE),
seed = 99
)
# Interaction between area and temperature but common squared term
m3 <- brm(A ~ area_f * temp_sc + temp_sq_sc,
data = dat,
cores = 4,
chains = 4,
iter = 4000,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE),
seed = 99
)
# Quadratic effect of temperature, full random
m4 <- brm(A ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f),
data = dat,
cores = 4,
chains = 4,
iter = 4000,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE),
seed = 99
)
# random intercept and slope?
m5 <- brm(A ~ temp_sc + (1 + temp_sc | area_f),
data = dat,
cores = 4,
chains = 4,
iter = 4000,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE),
control = list(adapt_delta = 0.95),
seed = 99
)Compare all models with loo
loo(m1, m2, m3, m4, m5,
moment_match = TRUE)Output of model 'm1':
Computed from 8000 by 306 log-likelihood matrix.
Estimate SE
elpd_loo -886.9 16.6
p_loo 13.8 0.9
looic 1773.7 33.1
------
MCSE of elpd_loo is 0.0.
MCSE and ESS estimates assume MCMC draws (r_eff in [0.3, 1.7]).
All Pareto k estimates are good (k < 0.7).
See help('pareto-k-diagnostic') for details.
Output of model 'm2':
Computed from 8000 by 306 log-likelihood matrix.
Estimate SE
elpd_loo -880.8 16.7
p_loo 20.5 1.7
looic 1761.6 33.4
------
MCSE of elpd_loo is 0.1.
MCSE and ESS estimates assume MCMC draws (r_eff in [0.3, 1.7]).
All Pareto k estimates are good (k < 0.7).
See help('pareto-k-diagnostic') for details.
Output of model 'm3':
Computed from 8000 by 306 log-likelihood matrix.
Estimate SE
elpd_loo -881.6 16.7
p_loo 21.5 1.8
looic 1763.2 33.5
------
MCSE of elpd_loo is 0.1.
MCSE and ESS estimates assume MCMC draws (r_eff in [0.4, 1.8]).
All Pareto k estimates are good (k < 0.7).
See help('pareto-k-diagnostic') for details.
Output of model 'm4':
Computed from 8000 by 306 log-likelihood matrix.
Estimate SE
elpd_loo -881.7 16.7
p_loo 21.2 2.2
looic 1763.3 33.5
------
MCSE of elpd_loo is 0.1.
MCSE and ESS estimates assume MCMC draws (r_eff in [0.2, 1.0]).
All Pareto k estimates are good (k < 0.7).
See help('pareto-k-diagnostic') for details.
Output of model 'm5':
Computed from 8000 by 306 log-likelihood matrix.
Estimate SE
elpd_loo -881.0 16.7
p_loo 17.8 1.6
looic 1762.1 33.4
------
MCSE of elpd_loo is 0.1.
MCSE and ESS estimates assume MCMC draws (r_eff in [0.5, 1.5]).
All Pareto k estimates are good (k < 0.7).
See help('pareto-k-diagnostic') for details.
Model comparisons:
elpd_diff se_diff
m2 0.0 0.0
m5 -0.2 2.1
m3 -0.8 0.5
m4 -0.9 2.4
m1 -6.1 4.1 Plot the favorite model
performance::r2_bayes(m4)# Bayesian R2 with Compatibility Interval
Conditional R2: 0.270 (95% CI [0.200, 0.338])
Marginal R2: 0.286 (95% CI [0.071, 0.514])prior_summary(m4) prior class coef group resp dpar nlpar lb ub
normal(0,5) b
normal(0,5) b temp_sc
normal(0,5) b temp_sq_sc
student_t(3, 43.4, 4.4) Intercept
lkj_corr_cholesky(1) L
lkj_corr_cholesky(1) L area_f
gamma(2, 0.1) nu 1
student_t(3, 0, 4.4) sd 0
student_t(3, 0, 4.4) sd area_f 0
student_t(3, 0, 4.4) sd Intercept area_f 0
student_t(3, 0, 4.4) sd temp_sc area_f 0
student_t(3, 0, 4.4) sd temp_sq_sc area_f 0
student_t(3, 0, 4.4) sigma 0
source
user
(vectorized)
(vectorized)
default
default
(vectorized)
default
default
(vectorized)
(vectorized)
(vectorized)
(vectorized)
defaultm4Warning: There were 2 divergent transitions after warmup. Increasing
adapt_delta above 0.8 may help. See
http://mc-stan.org/misc/warnings.html#divergent-transitions-after-warmup Family: student
Links: mu = identity; sigma = identity; nu = identity
Formula: A ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f)
Data: dat (Number of observations: 306)
Draws: 4 chains, each with iter = 4000; warmup = 2000; thin = 1;
total post-warmup draws = 8000
Group-Level Effects:
~area_f (Number of levels: 10)
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS
sd(Intercept) 1.80 0.79 0.55 3.61 1.00 1956
sd(temp_sc) 2.20 0.93 0.72 4.41 1.00 1065
sd(temp_sq_sc) 1.16 0.80 0.07 3.12 1.00 1511
cor(Intercept,temp_sc) 0.46 0.34 -0.35 0.93 1.00 2947
cor(Intercept,temp_sq_sc) 0.19 0.44 -0.70 0.91 1.00 4581
cor(temp_sc,temp_sq_sc) 0.45 0.42 -0.59 0.96 1.00 3515
Tail_ESS
sd(Intercept) 2951
sd(temp_sc) 625
sd(temp_sq_sc) 682
cor(Intercept,temp_sc) 3097
cor(Intercept,temp_sq_sc) 4149
cor(temp_sc,temp_sq_sc) 4534
Population-Level Effects:
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
Intercept 44.00 0.71 42.52 45.38 1.00 3503 4669
temp_sc 3.06 1.01 1.08 5.21 1.00 1597 589
temp_sq_sc 0.00 0.65 -1.29 1.49 1.00 1512 554
Family Specific Parameters:
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
sigma 3.40 0.27 2.88 3.95 1.00 2647 706
nu 5.91 2.47 3.14 11.95 1.00 3846 4230
Draws were sampled using sampling(NUTS). For each parameter, Bulk_ESS
and Tail_ESS are effective sample size measures, and Rhat is the potential
scale reduction factor on split chains (at convergence, Rhat = 1).# Predict with the main model
m4_pred <- dat %>%
group_by(area) %>%
data_grid(temp_sc = seq_range(temp_sc, n = 100)) %>%
ungroup() %>%
mutate(area_f = as.factor(area),
temp = (temp_sc * sd(VBG$temp)) + mean(VBG$temp),
temp_sq_sc = temp_sc*temp_sc) %>%
add_epred_draws(m4)
# Global prediction?
m4_pred_glob <- dat %>%
data_grid(temp_sc = seq_range(temp_sc, n = 100)) %>%
mutate(temp = (temp_sc * sd(VBG$temp)) + mean(VBG$temp),
temp_sq_sc = temp_sc*temp_sc) %>%
add_epred_draws(m4, re_formula = NA)
# Plot area specific predictions as facets
p0 <- scatter +
# scale_color_viridis(discrete = TRUE) +
# scale_fill_viridis(discrete = TRUE) +
stat_lineribbon(data = m4_pred,
aes(temp, y = .epred,
fill = factor(area, order$area)),
alpha = 0.1, .width = 0.95, linewidth = 0.9) +
stat_lineribbon(data = m4_pred,
aes(temp, y = .epred,
fill = factor(area, order$area)),
alpha = 1, .width = 0, linewidth = 0.9) +
# stat_lineribbon(data = m4_pred_glob,
# aes(temp, y = .epred), inherit.aes = FALSE, color = "grey30", fill = "grey30",
# alpha = 1, .width = 0, linewidth = 0.9) +
guides(fill = "none", color = guide_legend(nrow = 1, reverse = TRUE,
title.position = "top", title.hjust = 0.5,
override.aes = list(fill = NA),
position = "inside")) +
theme(legend.position.inside = c(0.5, 0.05),
axis.title.y = ggtext::element_markdown()) +
labs(x = "Mean site temperature [°C]", y = "Size-corrected growth coefficient (*A*)",
color = "")
p0
ggsave(paste0(home, "/figures/a_conditional.pdf" ), width = 17, height = 17, units = "cm")
# Facet
# scatter +
# stat_lineribbon(data = m5_pred,
# aes(temp, y = .epred,
# fill = factor(area, order$area)),
# alpha = 0.1, .width = 0.95) +
# stat_lineribbon(data = m5_pred,
# aes(temp, y = .epred,
# fill = factor(area, order$area)),
# alpha = 1, .width = 0) +
# facet_wrap(~area)
#
# ggsave(paste0(home, "/figures/supp/A_by_area.pdf" ), width = 17, height = 17, units = "cm")
# Look at the slopes and intercepts by area
mean_temps <- pred_temp %>%
group_by(area) %>%
summarise(mean_temp = mean(temp))
# p1 <- m4 %>%
# spread_draws(b_temp_sc, r_area_f[temp_sc,]) %>%
# median_qi(slope = b_temp_sc + r_area_f, .width = c(.9)) %>%
# mutate(area = temp_sc) %>%
# left_join(mean_temps, by = "area") %>%
# ggplot(aes(y = slope, x = mean_temp, ymin = .lower, ymax = .upper,
# color = factor(area, order$area),
# fill = factor(area, order$area))) +
# geom_hline(yintercept = 0, linetype = 2, alpha = 0.5, linewidth = 0.8) +
# geom_smooth(method = "lm", inherit.aes = FALSE,
# aes(mean_temp, slope), alpha = 0.15, color = "grey") +
# geom_point() +
# geom_errorbar(alpha = 0.3) +
# scale_color_manual(values = colors, name = "Site") +
# scale_fill_manual(values = colors, name = "Site") +
# labs(y = "Site specific slope", x = "Mean site temperature (°C)") +
# guides(fill = "none",
# color = "none") +
# theme(axis.title.y = ggtext::element_markdown())
#
p2 <- m4 %>%
spread_draws(b_Intercept, r_area_f[Intercept,]) %>%
median_qi(intercept = b_Intercept + r_area_f, .width = c(.9)) %>%
mutate(area = Intercept) %>%
left_join(mean_temps, by = "area") %>%
ggplot(aes(y = intercept, x = mean_temp, ymin = .lower, ymax = .upper,
color = factor(area, order$area),
fill = factor(area, order$area))) +
geom_smooth(method = "lm", inherit.aes = FALSE, linetype = 2,
aes(mean_temp, intercept), alpha = 0.15, color = "grey") +
geom_point(size = 2) +
geom_errorbar(alpha = 0.3) +
scale_color_manual(values = colors, name = "Site") +
scale_fill_manual(values = colors, name = "Site") +
labs(y = "Site-specific intercept", x = "Mean site temperature (°C)") +
guides(fill = "none",
color = "none") +
theme(axis.title.y = ggtext::element_markdown())
p2 
ggsave(paste0(home, "/figures/random_intercepts.pdf" ), width = 11, height = 11, units = "cm")
# p0 / ((p2 + p1) + plot_layout(axes = "collect")) +
# plot_annotation(tag_level = "A") +
# plot_layout(heights = c(2, 1))
#
# ggsave(paste0(home, "/figures/a_conditional.pdf" ), width = 17, height = 20, units = "cm")
# Check significance of slopes?!
# get_variables(m5)
# t_inter <- m5 %>%
# spread_draws(b_Intercept, r_area_f[Intercept,]) %>%
# median_qi(intercept = b_Intercept + r_area_f, .width = c(.9)) %>%
# mutate(area = Intercept) %>%
# left_join(mean_temps, by = "area")
#
# t_slope <- m5 %>%
# spread_draws(b_temp_sc, r_area_f[temp_sc,]) %>%
# median_qi(slope = b_temp_sc + r_area_f, .width = c(.9)) %>%
# mutate(area = temp_sc) %>%
# left_join(mean_temps, by = "area")
#
# tidy(lm(intercept ~ mean_temp, data = t_inter))
# tidy(lm(slope ~ mean_temp, data = t_slope))Plot conceptual model
get_variables(m4) [1] "b_Intercept" "b_temp_sc"
[3] "b_temp_sq_sc" "sd_area_f__Intercept"
[5] "sd_area_f__temp_sc" "sd_area_f__temp_sq_sc"
[7] "cor_area_f__Intercept__temp_sc" "cor_area_f__Intercept__temp_sq_sc"
[9] "cor_area_f__temp_sc__temp_sq_sc" "sigma"
[11] "nu" "Intercept"
[13] "r_area_f[BS,Intercept]" "r_area_f[BT,Intercept]"
[15] "r_area_f[FB,Intercept]" "r_area_f[FM,Intercept]"
[17] "r_area_f[HO,Intercept]" "r_area_f[JM,Intercept]"
[19] "r_area_f[MU,Intercept]" "r_area_f[RA,Intercept]"
[21] "r_area_f[SI_EK,Intercept]" "r_area_f[SI_HA,Intercept]"
[23] "r_area_f[BS,temp_sc]" "r_area_f[BT,temp_sc]"
[25] "r_area_f[FB,temp_sc]" "r_area_f[FM,temp_sc]"
[27] "r_area_f[HO,temp_sc]" "r_area_f[JM,temp_sc]"
[29] "r_area_f[MU,temp_sc]" "r_area_f[RA,temp_sc]"
[31] "r_area_f[SI_EK,temp_sc]" "r_area_f[SI_HA,temp_sc]"
[33] "r_area_f[BS,temp_sq_sc]" "r_area_f[BT,temp_sq_sc]"
[35] "r_area_f[FB,temp_sq_sc]" "r_area_f[FM,temp_sq_sc]"
[37] "r_area_f[HO,temp_sq_sc]" "r_area_f[JM,temp_sq_sc]"
[39] "r_area_f[MU,temp_sq_sc]" "r_area_f[RA,temp_sq_sc]"
[41] "r_area_f[SI_EK,temp_sq_sc]" "r_area_f[SI_HA,temp_sq_sc]"
[43] "lprior" "lp__"
[45] "z_1[1,1]" "z_1[2,1]"
[47] "z_1[3,1]" "z_1[1,2]"
[49] "z_1[2,2]" "z_1[3,2]"
[51] "z_1[1,3]" "z_1[2,3]"
[53] "z_1[3,3]" "z_1[1,4]"
[55] "z_1[2,4]" "z_1[3,4]"
[57] "z_1[1,5]" "z_1[2,5]"
[59] "z_1[3,5]" "z_1[1,6]"
[61] "z_1[2,6]" "z_1[3,6]"
[63] "z_1[1,7]" "z_1[2,7]"
[65] "z_1[3,7]" "z_1[1,8]"
[67] "z_1[2,8]" "z_1[3,8]"
[69] "z_1[1,9]" "z_1[2,9]"
[71] "z_1[3,9]" "z_1[1,10]"
[73] "z_1[2,10]" "z_1[3,10]"
[75] "L_1[1,1]" "L_1[2,1]"
[77] "L_1[3,1]" "L_1[1,2]"
[79] "L_1[2,2]" "L_1[3,2]"
[81] "L_1[1,3]" "L_1[2,3]"
[83] "L_1[3,3]" "accept_stat__"
[85] "stepsize__" "treedepth__"
[87] "n_leapfrog__" "divergent__"
[89] "energy__" par <- m4 %>%
spread_draws(b_Intercept, b_temp_sc, b_temp_sq_sc,
`r_area_f[FM,Intercept]`, `r_area_f[FM,temp_sc]`, `r_area_f[FM,temp_sq_sc]`) %>%
ungroup() %>%
mutate(intercept = b_Intercept + `r_area_f[FM,Intercept]`,
b1 = b_temp_sc + `r_area_f[FM,temp_sc]`,
b2 = b_temp_sq_sc + `r_area_f[FM,temp_sq_sc]`) %>%
summarise(intercept = mean(intercept),
b1 = mean(b1),
b2 = mean(b2))ungroup: no grouping variablesmutate: new variable 'intercept' (double) with 7,885 unique values and 0% NA new variable 'b1' (double) with 7,885 unique values and 0% NA new variable 'b2' (double) with 7,885 unique values and 0% NAsummarise: now one row and 3 columns, ungrouped# No adaptation
no_adapt_1 <- tibble(temp = seq(5, 15, length.out = 500)) %>%
mutate(temp_sc = (temp - mean(VBG$temp) / sd(VBG$temp)),
temp_sc_sq = temp_sc*temp_sc) %>%
mutate(growth_rate = par$intercept + temp_sc*(par$b1 * 3) + temp_sc_sq*(par$b2*0.08),
pop = "Cold")mutate: new variable 'temp_sc' (double) with 500 unique values and 0% NA new variable 'temp_sc_sq' (double) with 500 unique values and 0% NAmutate: new variable 'growth_rate' (double) with 500 unique values and 0% NA new variable 'pop' (character) with one unique value and 0% NAno_adapt_2 <- tibble(temp = seq(10, 20, length.out = 500)) %>%
mutate(temp_sc = (temp - mean(VBG$temp) / sd(VBG$temp)),
temp_sc_sq = temp_sc*temp_sc) %>%
mutate(growth_rate = par$intercept + temp_sc*(par$b1 * 3) + temp_sc_sq*(par$b2*0.08),
pop = "Medium",
growth_rate = growth_rate + 0.5)mutate: new variable 'temp_sc' (double) with 500 unique values and 0% NA new variable 'temp_sc_sq' (double) with 500 unique values and 0% NAmutate: new variable 'growth_rate' (double) with 500 unique values and 0% NA new variable 'pop' (character) with one unique value and 0% NAno_adapt_3 <- tibble(temp = seq(15, 25, length.out = 500)) %>%
mutate(temp_sc = (temp - mean(VBG$temp) / sd(VBG$temp)),
temp_sc_sq = temp_sc*temp_sc) %>%
mutate(growth_rate = par$intercept + temp_sc*(par$b1 * 3) + temp_sc_sq*(par$b2*0.08),
pop = "Warm",
growth_rate = growth_rate + 1)mutate: new variable 'temp_sc' (double) with 500 unique values and 0% NA new variable 'temp_sc_sq' (double) with 500 unique values and 0% NAmutate: new variable 'growth_rate' (double) with 500 unique values and 0% NA new variable 'pop' (character) with one unique value and 0% NAno_adapt <- bind_rows(no_adapt_1, no_adapt_2, no_adapt_3)
p1 <- ggplot(no_adapt, aes(temp, growth_rate, color = pop)) +
geom_line(linewidth = 1.2, alpha = 0.8) +
labs(tag = "No adaptation", y = "Increasing growth rate →", x = "Increasing temperature →", color = "Site") +
theme(plot.tag = element_text(size = 9),
plot.tag.position = c(0.23, 0.94),
legend.position.inside = c(0.5, 0.1),
axis.text.y = element_blank(),
axis.ticks.y = element_blank(),
axis.text.x = element_blank(),
axis.ticks.x = element_blank(),
legend.direction = "horizontal") +
guides(color = guide_legend(position = "inside", title.position = "top", title.hjust = 0.5))
p1
# Now do "local adaption
adapt_1 <- tibble(temp = seq(5, 15, length.out = 500)) %>%
mutate(temp_sc = (temp - mean(VBG$temp) / sd(VBG$temp)),
temp_sc_sq = temp_sc*temp_sc) %>%
mutate(growth_rate = par$intercept + temp_sc*(par$b1 * 4) + temp_sc_sq*(par$b2*0.18),
pop = "Cold")mutate: new variable 'temp_sc' (double) with 500 unique values and 0% NA new variable 'temp_sc_sq' (double) with 500 unique values and 0% NAmutate: new variable 'growth_rate' (double) with 500 unique values and 0% NA new variable 'pop' (character) with one unique value and 0% NAadapt_2 <- tibble(temp = seq(10, 20, length.out = 500)) %>%
mutate(temp_sc = (temp - mean(VBG$temp) / sd(VBG$temp)),
temp_sc_sq = temp_sc*temp_sc) %>%
#mutate(growth_rate = par$intercept*0.95 + temp_sc*(par$b1 * 3.5) + temp_sc_sq*(par$b2*0.1),
mutate(growth_rate = par$intercept*0.976 + temp_sc*(par$b1 * 3) + temp_sc_sq*(par$b2*0.08),
pop = "Medium",
growth_rate = growth_rate + 1)mutate: new variable 'temp_sc' (double) with 500 unique values and 0% NA new variable 'temp_sc_sq' (double) with 500 unique values and 0% NAmutate: new variable 'growth_rate' (double) with 500 unique values and 0% NA new variable 'pop' (character) with one unique value and 0% NAadapt_3 <- tibble(temp = seq(15, 25, length.out = 500)) %>%
mutate(temp_sc = (temp - mean(VBG$temp) / sd(VBG$temp)),
temp_sc_sq = temp_sc*temp_sc) %>%
mutate(growth_rate = par$intercept*0.715 + temp_sc*(par$b1 * 5) + temp_sc_sq*(par$b2*0.11),
pop = "Warm",
growth_rate = growth_rate + 2)mutate: new variable 'temp_sc' (double) with 500 unique values and 0% NA new variable 'temp_sc_sq' (double) with 500 unique values and 0% NAmutate: new variable 'growth_rate' (double) with 500 unique values and 0% NA new variable 'pop' (character) with one unique value and 0% NA# ggplot(adapt_3, aes(temp, growth_rate, color = pop)) +
# geom_line(linewidth = 1.2, alpha = 0.8)
adapt <- bind_rows(adapt_1, adapt_2, adapt_3)
p2 <-
ggplot(adapt, aes(temp, growth_rate, color = pop)) +
geom_line(linewidth = 1.2, alpha = 0.8) +
labs(tag = "Local adaptation", y = "Increasing growth rate →", x = "Increasing temperature →") +
theme(plot.tag = element_text(size = 9),
plot.tag.position = c(0.2, 0.94),
axis.text.y = element_blank(),
axis.ticks.y = element_blank(),
axis.text.x = element_blank(),
axis.ticks.x = element_blank()) +
guides(color = "none")
p1 + p2 +
plot_layout(axes = "collect") &
scale_color_manual(values = colors[c(10, 5, 1)])
ggsave(paste0(home, "/figures/concept.pdf"), width = 17, height = 8, units = "cm", device = cairo_pdf)Supplementary plot
# Plot prior vs posterior
# Refit with sampling on prior
ggsave(paste0(home, "/figures/supp/pp_check.pdf" ), width = 11, height = 11, units = "cm")Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call(C_textBounds, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing growth rate →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <92>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <e2>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <86>Warning in grid.Call.graphics(C_text, as.graphicsAnnot(x$label), x$x, x$y, :
conversion failure on 'Increasing temperature →' in 'mbcsToSbcs': dot
substituted for <92>m4p <- brm(A ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f),
data = dat,
cores = 4,
chains = 4,
iter = 4000,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
sample_prior = TRUE,
save_pars = save_pars(all = TRUE),
control = list(adapt_delta = 0.95),
)Compiling Stan program...Trying to compile a simple C fileRunning /Library/Frameworks/R.framework/Resources/bin/R CMD SHLIB foo.c
using C compiler: ‘Apple clang version 15.0.0 (clang-1500.1.0.2.5)’
using SDK: ‘MacOSX14.2.sdk’
clang -arch arm64 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/Rcpp/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/unsupported" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/BH/include" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/src/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppParallel/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/rstan/include" -DEIGEN_NO_DEBUG -DBOOST_DISABLE_ASSERTS -DBOOST_PENDING_INTEGER_LOG2_HPP -DSTAN_THREADS -DUSE_STANC3 -DSTRICT_R_HEADERS -DBOOST_PHOENIX_NO_VARIADIC_EXPRESSION -D_HAS_AUTO_PTR_ETC=0 -include '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/stan/math/prim/fun/Eigen.hpp' -D_REENTRANT -DRCPP_PARALLEL_USE_TBB=1 -I/opt/R/arm64/include -fPIC -falign-functions=64 -Wall -g -O2 -c foo.c -o foo.o
In file included from <built-in>:1:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/stan/math/prim/fun/Eigen.hpp:22:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/Dense:1:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/Core:19:
/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/src/Core/util/Macros.h:679:10: fatal error: 'cmath' file not found
#include <cmath>
^~~~~~~
1 error generated.
make: *** [foo.o] Error 1Start samplingprior_summary(m4p) prior class coef group resp dpar nlpar lb ub
normal(0,5) b
normal(0,5) b temp_sc
normal(0,5) b temp_sq_sc
student_t(3, 43.4, 4.4) Intercept
lkj_corr_cholesky(1) L
lkj_corr_cholesky(1) L area_f
gamma(2, 0.1) nu 1
student_t(3, 0, 4.4) sd 0
student_t(3, 0, 4.4) sd area_f 0
student_t(3, 0, 4.4) sd Intercept area_f 0
student_t(3, 0, 4.4) sd temp_sc area_f 0
student_t(3, 0, 4.4) sd temp_sq_sc area_f 0
student_t(3, 0, 4.4) sigma 0
source
user
(vectorized)
(vectorized)
default
default
(vectorized)
default
default
(vectorized)
(vectorized)
(vectorized)
(vectorized)
defaultget_variables(m4p) [1] "b_Intercept" "b_temp_sc"
[3] "b_temp_sq_sc" "sd_area_f__Intercept"
[5] "sd_area_f__temp_sc" "sd_area_f__temp_sq_sc"
[7] "cor_area_f__Intercept__temp_sc" "cor_area_f__Intercept__temp_sq_sc"
[9] "cor_area_f__temp_sc__temp_sq_sc" "sigma"
[11] "nu" "Intercept"
[13] "r_area_f[BS,Intercept]" "r_area_f[BT,Intercept]"
[15] "r_area_f[FB,Intercept]" "r_area_f[FM,Intercept]"
[17] "r_area_f[HO,Intercept]" "r_area_f[JM,Intercept]"
[19] "r_area_f[MU,Intercept]" "r_area_f[RA,Intercept]"
[21] "r_area_f[SI_EK,Intercept]" "r_area_f[SI_HA,Intercept]"
[23] "r_area_f[BS,temp_sc]" "r_area_f[BT,temp_sc]"
[25] "r_area_f[FB,temp_sc]" "r_area_f[FM,temp_sc]"
[27] "r_area_f[HO,temp_sc]" "r_area_f[JM,temp_sc]"
[29] "r_area_f[MU,temp_sc]" "r_area_f[RA,temp_sc]"
[31] "r_area_f[SI_EK,temp_sc]" "r_area_f[SI_HA,temp_sc]"
[33] "r_area_f[BS,temp_sq_sc]" "r_area_f[BT,temp_sq_sc]"
[35] "r_area_f[FB,temp_sq_sc]" "r_area_f[FM,temp_sq_sc]"
[37] "r_area_f[HO,temp_sq_sc]" "r_area_f[JM,temp_sq_sc]"
[39] "r_area_f[MU,temp_sq_sc]" "r_area_f[RA,temp_sq_sc]"
[41] "r_area_f[SI_EK,temp_sq_sc]" "r_area_f[SI_HA,temp_sq_sc]"
[43] "prior_Intercept" "prior_b"
[45] "prior_sigma" "prior_nu"
[47] "prior_sd_area_f" "prior_cor_area_f"
[49] "lprior" "lp__"
[51] "z_1[1,1]" "z_1[2,1]"
[53] "z_1[3,1]" "z_1[1,2]"
[55] "z_1[2,2]" "z_1[3,2]"
[57] "z_1[1,3]" "z_1[2,3]"
[59] "z_1[3,3]" "z_1[1,4]"
[61] "z_1[2,4]" "z_1[3,4]"
[63] "z_1[1,5]" "z_1[2,5]"
[65] "z_1[3,5]" "z_1[1,6]"
[67] "z_1[2,6]" "z_1[3,6]"
[69] "z_1[1,7]" "z_1[2,7]"
[71] "z_1[3,7]" "z_1[1,8]"
[73] "z_1[2,8]" "z_1[3,8]"
[75] "z_1[1,9]" "z_1[2,9]"
[77] "z_1[3,9]" "z_1[1,10]"
[79] "z_1[2,10]" "z_1[3,10]"
[81] "L_1[1,1]" "L_1[2,1]"
[83] "L_1[3,1]" "L_1[1,2]"
[85] "L_1[2,2]" "L_1[3,2]"
[87] "L_1[1,3]" "L_1[2,3]"
[89] "L_1[3,3]" "accept_stat__"
[91] "stepsize__" "treedepth__"
[93] "n_leapfrog__" "divergent__"
[95] "energy__" plot(m4)
post_draws <- get_post_draws(model = m4p,
params = c("b_Intercept", "b_temp_sc", "sigma",
"nu"))Warning: Dropping 'draws_df' class as required metadata was removed.pivot_longer: reorganized (b_Intercept, b_temp_sc, sigma, nu) into (parameter, value) [was 8000x4, now 32000x2]mutate: new variable 'type' (character) with one unique value and 0% NAprior_draws <- get_prior_draws(model = m4p,
params = c("prior_Intercept", "prior_b", "prior_sigma",
"prior_nu"))Warning: Dropping 'draws_df' class as required metadata was removed.pivot_longer: reorganized (prior_Intercept, prior_b, prior_sigma, prior_nu) into (parameter, value) [was 8000x4, now 32000x2]mutate: changed 32,000 values (100%) of 'parameter' (0 new NA)mutate: new variable 'type' (character) with one unique value and 0% NAdist <- bind_rows(post_draws %>%
mutate(parameter = ifelse(parameter == "b_Intercept",
"Intercept",
parameter),
parameter = ifelse(parameter == "b_temp_sc",
"temp_sc",
parameter)),
prior_draws %>%
mutate(parameter = ifelse(parameter == "b", "temp_sc",
parameter))
)mutate: changed 16,000 values (50%) of 'parameter' (0 new NA)mutate: changed 8,000 values (25%) of 'parameter' (0 new NA)plot_prior_post(dist, type) +
theme(legend.position.inside = c(0.93, 0.97)) +
guides(fill = guide_legend(position = "inside")) +
labs(x = "Value", y = "Density") +
facet_wrap(~parameter, scales = "free")Warning: A numeric `legend.position` argument in `theme()` was deprecated in ggplot2
3.5.0.
ℹ Please use the `legend.position.inside` argument of `theme()` instead.
ggsave(paste0(home, "/figures/supp/brms_prior.pdf" ), width = 17, height = 17, units = "cm")
# Posterior predictive
pp_check(m4p) +
theme(legend.position.inside = c(0.8, 0.8)) +
guides(color = guide_legend(position = "inside")) +
labs(x = "Growth coefficient (*A*)")Using 10 posterior draws for ppc type 'dens_overlay' by default.
Fit models of K and Linf to temperature
# Quadratic effect of temperature
m4k <- brm(k ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f),
data = dat,
cores = 4,
chains = 4,
iter = 4000,
seed = 99,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE),
control = list(adapt_delta = 0.999),
)Compiling Stan program...Trying to compile a simple C fileRunning /Library/Frameworks/R.framework/Resources/bin/R CMD SHLIB foo.c
using C compiler: ‘Apple clang version 15.0.0 (clang-1500.1.0.2.5)’
using SDK: ‘MacOSX14.2.sdk’
clang -arch arm64 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/Rcpp/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/unsupported" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/BH/include" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/src/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppParallel/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/rstan/include" -DEIGEN_NO_DEBUG -DBOOST_DISABLE_ASSERTS -DBOOST_PENDING_INTEGER_LOG2_HPP -DSTAN_THREADS -DUSE_STANC3 -DSTRICT_R_HEADERS -DBOOST_PHOENIX_NO_VARIADIC_EXPRESSION -D_HAS_AUTO_PTR_ETC=0 -include '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/stan/math/prim/fun/Eigen.hpp' -D_REENTRANT -DRCPP_PARALLEL_USE_TBB=1 -I/opt/R/arm64/include -fPIC -falign-functions=64 -Wall -g -O2 -c foo.c -o foo.o
In file included from <built-in>:1:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/stan/math/prim/fun/Eigen.hpp:22:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/Dense:1:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/Core:19:
/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/src/Core/util/Macros.h:679:10: fatal error: 'cmath' file not found
#include <cmath>
^~~~~~~
1 error generated.
make: *** [foo.o] Error 1Start samplingprior_summary(m4k) prior class coef group resp dpar nlpar lb ub
normal(0,5) b
normal(0,5) b temp_sc
normal(0,5) b temp_sq_sc
student_t(3, 0.2, 2.5) Intercept
lkj_corr_cholesky(1) L
lkj_corr_cholesky(1) L area_f
gamma(2, 0.1) nu 1
student_t(3, 0, 2.5) sd 0
student_t(3, 0, 2.5) sd area_f 0
student_t(3, 0, 2.5) sd Intercept area_f 0
student_t(3, 0, 2.5) sd temp_sc area_f 0
student_t(3, 0, 2.5) sd temp_sq_sc area_f 0
student_t(3, 0, 2.5) sigma 0
source
user
(vectorized)
(vectorized)
default
default
(vectorized)
default
default
(vectorized)
(vectorized)
(vectorized)
(vectorized)
defaultm4l <- brm(linf ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f),
data = dat,
cores = 4,
chains = 4,
iter = 4000,
seed = 99,
family = student,
prior = set_prior("normal(0,5)", class = "b"),
save_pars = save_pars(all = TRUE)
)Compiling Stan program...Trying to compile a simple C fileRunning /Library/Frameworks/R.framework/Resources/bin/R CMD SHLIB foo.c
using C compiler: ‘Apple clang version 15.0.0 (clang-1500.1.0.2.5)’
using SDK: ‘MacOSX14.2.sdk’
clang -arch arm64 -I"/Library/Frameworks/R.framework/Resources/include" -DNDEBUG -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/Rcpp/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/unsupported" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/BH/include" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/src/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppParallel/include/" -I"/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/rstan/include" -DEIGEN_NO_DEBUG -DBOOST_DISABLE_ASSERTS -DBOOST_PENDING_INTEGER_LOG2_HPP -DSTAN_THREADS -DUSE_STANC3 -DSTRICT_R_HEADERS -DBOOST_PHOENIX_NO_VARIADIC_EXPRESSION -D_HAS_AUTO_PTR_ETC=0 -include '/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/stan/math/prim/fun/Eigen.hpp' -D_REENTRANT -DRCPP_PARALLEL_USE_TBB=1 -I/opt/R/arm64/include -fPIC -falign-functions=64 -Wall -g -O2 -c foo.c -o foo.o
In file included from <built-in>:1:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/StanHeaders/include/stan/math/prim/fun/Eigen.hpp:22:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/Dense:1:
In file included from /Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/Core:19:
/Library/Frameworks/R.framework/Versions/4.3-arm64/Resources/library/RcppEigen/include/Eigen/src/Core/util/Macros.h:679:10: fatal error: 'cmath' file not found
#include <cmath>
^~~~~~~
1 error generated.
make: *** [foo.o] Error 1Start samplingprior_summary(m4l) prior class coef group resp dpar nlpar lb ub
normal(0,5) b
normal(0,5) b temp_sc
normal(0,5) b temp_sq_sc
student_t(3, 340.9, 80) Intercept
lkj_corr_cholesky(1) L
lkj_corr_cholesky(1) L area_f
gamma(2, 0.1) nu 1
student_t(3, 0, 80) sd 0
student_t(3, 0, 80) sd area_f 0
student_t(3, 0, 80) sd Intercept area_f 0
student_t(3, 0, 80) sd temp_sc area_f 0
student_t(3, 0, 80) sd temp_sq_sc area_f 0
student_t(3, 0, 80) sigma 0
source
user
(vectorized)
(vectorized)
default
default
(vectorized)
default
default
(vectorized)
(vectorized)
(vectorized)
(vectorized)
defaultPlot these fits
# PP_check
p1 <- pp_check(m4k) +
theme(legend.position.inside = c(0.8, 0.8),
axis.title.x = element_markdown()) +
guides(color = guide_legend(position = "inside")) +
labs(x = "*k*")Using 10 posterior draws for ppc type 'dens_overlay' by default.p2 <- pp_check(m4l) +
coord_cartesian(xlim = c(0, 1000)) +
theme(legend.position.inside = c(0.8, 0.8)) +
guides(color = guide_legend(position = "inside")) +
labs(x = expression(paste(italic(L[infinity]), " [mm]")))Using 10 posterior draws for ppc type 'dens_overlay' by default.Coordinate system already present. Adding new coordinate system, which will
replace the existing one.p1 + p2
ggsave(paste0(home, "/figures/supp/k_linf_pp_check.pdf" ), width = 17, height = 11, units = "cm")
# Conditional predictions
m4k_pred <- dat %>%
group_by(area) %>%
data_grid(temp_sc = seq_range(temp_sc, n = 100)) %>%
ungroup() %>%
mutate(area_f = as.factor(area),
temp_sq_sc = temp_sc*temp_sc,
temp = (temp_sc * sd(VBG$temp)) + mean(VBG$temp)) %>%
add_epred_draws(m4k)group_by: one grouping variable (area)
ungroup: no grouping variables
mutate: new variable 'area_f' (factor) with 10 unique values and 0% NA
new variable 'temp_sq_sc' (double) with 999 unique values and 0% NA
new variable 'temp' (double) with 999 unique values and 0% NAm4l_pred <- dat %>%
group_by(area) %>%
data_grid(temp_sc = seq_range(temp_sc, n = 100)) %>%
ungroup() %>%
mutate(area_f = as.factor(area),
temp_sq_sc = temp_sc*temp_sc,
temp = (temp_sc * sd(VBG$temp)) + mean(VBG$temp)) %>%
add_epred_draws(m4l)group_by: one grouping variable (area)
ungroup: no grouping variables
mutate: new variable 'area_f' (factor) with 10 unique values and 0% NA
new variable 'temp_sq_sc' (double) with 999 unique values and 0% NA
new variable 'temp' (double) with 999 unique values and 0% NA#m4_kl <- bind_rows(m4k_pred, m4l_pred)
# K
pk <- ggplot(dat, aes(temp, k, color = factor(area, order$area)), size = 0.6) +
geom_point() +
scale_color_manual(values = colors, name = "Site") +
scale_fill_manual(values = colors, name = "Site") +
guides(fill = "none",
color = guide_legend(nrow = 1, reverse = TRUE, title.position = "top", title.hjust = 0.5,
override.aes = list(size = 1))) +
theme(axis.title.y = ggtext::element_markdown(),
legend.position = "bottom",
legend.direction = "horizontal") +
stat_lineribbon(data = m4k_pred,
aes(temp, y = .epred,
fill = factor(area, order$area)),
alpha = 0.1, .width = 0.95) +
stat_lineribbon(data = m4k_pred,
aes(temp, y = .epred,
fill = factor(area, order$area)),
alpha = 1, .width = 0) +
guides(fill = "none", color = guide_legend(nrow = 1, reverse = TRUE,
title.position = "top", title.hjust = 0.5,
override.aes = list(fill = NA))) +
labs(x = "Mean site temperature [°C]", y = "*k*", color = "")
# Linf
pl <- ggplot(dat, aes(temp, linf, color = factor(area, order$area)), size = 0.6) +
geom_point() +
scale_color_manual(values = colors, name = "Site") +
scale_fill_manual(values = colors, name = "Site") +
theme(legend.position = "bottom",
legend.direction = "horizontal") +
stat_lineribbon(data = m4l_pred,
aes(temp, y = .epred,
fill = factor(area, order$area)),
alpha = 0.1, .width = 0.95) +
stat_lineribbon(data = m4l_pred,
aes(temp, y = .epred,
fill = factor(area, order$area)),
alpha = 1, .width = 0) +
guides(fill = "none", color = guide_legend(nrow = 1, reverse = TRUE,
title.position = "top", title.hjust = 0.5,
override.aes = list(fill = NA))) +
labs(x = "Mean site temperature [°C]", y = expression(paste(italic(L[infinity]), " [mm]")),
color = "")
pk / pl +
plot_layout(axis = "collect", guides = "collect") &
theme(legend.position = "bottom")
ggsave(paste0(home, "/figures/supp/k_linf_conditional.pdf" ), width = 17, height = 25, units = "cm")Print global temperature slopes
summary(m4k) Family: student
Links: mu = identity; sigma = identity; nu = identity
Formula: k ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f)
Data: dat (Number of observations: 306)
Draws: 4 chains, each with iter = 4000; warmup = 2000; thin = 1;
total post-warmup draws = 8000
Group-Level Effects:
~area_f (Number of levels: 10)
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS
sd(Intercept) 0.03 0.01 0.01 0.05 1.00 3097
sd(temp_sc) 0.02 0.01 0.00 0.04 1.00 1812
sd(temp_sq_sc) 0.01 0.01 0.00 0.02 1.00 2558
cor(Intercept,temp_sc) 0.14 0.41 -0.67 0.86 1.00 5520
cor(Intercept,temp_sq_sc) 0.01 0.49 -0.86 0.85 1.00 6136
cor(temp_sc,temp_sq_sc) -0.07 0.50 -0.89 0.86 1.00 5407
Tail_ESS
sd(Intercept) 4088
sd(temp_sc) 2862
sd(temp_sq_sc) 3626
cor(Intercept,temp_sc) 4955
cor(Intercept,temp_sq_sc) 4832
cor(temp_sc,temp_sq_sc) 5967
Population-Level Effects:
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
Intercept 0.20 0.01 0.18 0.22 1.00 2521 3550
temp_sc 0.00 0.01 -0.02 0.02 1.00 3561 3273
temp_sq_sc -0.01 0.01 -0.02 0.00 1.00 3554 3433
Family Specific Parameters:
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
sigma 0.04 0.00 0.04 0.04 1.00 7852 5735
nu 24.74 13.55 8.13 60.16 1.00 8558 6433
Draws were sampled using sampling(NUTS). For each parameter, Bulk_ESS
and Tail_ESS are effective sample size measures, and Rhat is the potential
scale reduction factor on split chains (at convergence, Rhat = 1).summary(m4l) Family: student
Links: mu = identity; sigma = identity; nu = identity
Formula: linf ~ temp_sc + temp_sq_sc + (1 + temp_sc + temp_sq_sc | area_f)
Data: dat (Number of observations: 306)
Draws: 4 chains, each with iter = 4000; warmup = 2000; thin = 1;
total post-warmup draws = 8000
Group-Level Effects:
~area_f (Number of levels: 10)
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS
sd(Intercept) 29.25 13.07 9.16 60.43 1.00 2870
sd(temp_sc) 52.76 18.25 24.40 95.43 1.00 3419
sd(temp_sq_sc) 14.58 10.27 0.65 39.15 1.00 2674
cor(Intercept,temp_sc) -0.41 0.34 -0.92 0.32 1.00 3192
cor(Intercept,temp_sq_sc) -0.10 0.48 -0.89 0.81 1.00 5364
cor(temp_sc,temp_sq_sc) 0.29 0.43 -0.65 0.92 1.00 7399
Tail_ESS
sd(Intercept) 3292
sd(temp_sc) 3666
sd(temp_sq_sc) 3629
cor(Intercept,temp_sc) 4256
cor(Intercept,temp_sq_sc) 4896
cor(temp_sc,temp_sq_sc) 5424
Population-Level Effects:
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
Intercept 335.60 12.17 313.32 361.76 1.00 3239 4081
temp_sc 0.34 4.84 -8.96 9.89 1.00 11563 5715
temp_sq_sc 5.11 4.58 -4.01 13.77 1.00 6537 4722
Family Specific Parameters:
Estimate Est.Error l-95% CI u-95% CI Rhat Bulk_ESS Tail_ESS
sigma 48.52 4.56 39.91 57.82 1.00 6300 4478
nu 2.55 0.53 1.73 3.78 1.00 6539 4752
Draws were sampled using sampling(NUTS). For each parameter, Bulk_ESS
and Tail_ESS are effective sample size measures, and Rhat is the potential
scale reduction factor on split chains (at convergence, Rhat = 1).Calculate VBGE fits for specific temperatures curves. Since the global effects of temperature are flat, we have to do it with area-specific predictions. But which ones? Here I predict k and Linf for 2 temperatures: mean in area and +3 °C. I then use those to calculate reference and warm VBGE curves. There’s a tendency for an increase in size-at-age in warmer areas. This we sort of see already in the plots of Linf against temperature by area, where cool populations decline and warm increase their Linf.
# Area specific parameters, then calculate growth curves from those
# For the plots below I trim age to 12, which is approximate maximum... with 40, we are essentially just plotting Linf, which we already have better plots for
age_bc <- 0:40
# Predict k across areas
m4k_pars <- dat %>%
group_by(area_f) %>%
data_grid(temp = mean(temp)) %>%
ungroup() %>%
mutate(temp2 = temp + 3) %>%
pivot_longer(c("temp2", "temp"), values_to = "temp") %>%
dplyr::select(-name) %>%
mutate(temp_sc = (temp - mean(dat$temp)) / sd(dat$temp),
temp_sq_sc = temp_sc*temp_sc) %>%
add_epred_draws(m4k) %>%
rename(k = .epred) %>%
group_by(temp, area_f) %>%
summarise(k_median = quantile(k, prob = 0.5),
k_lwr = quantile(k, prob = 0.05),
k_upr = quantile(k, prob = 0.95))group_by: one grouping variable (area_f)ungroup: no grouping variablesmutate: new variable 'temp2' (double) with 10 unique values and 0% NApivot_longer: reorganized (temp2) into (name) [was 10x3, now 20x3]mutate: new variable 'temp_sc' (double) with 20 unique values and 0% NA new variable 'temp_sq_sc' (double) with 20 unique values and 0% NArename: renamed one variable (k)group_by: 2 grouping variables (temp, area_f)summarise: now 20 rows and 5 columns, one group variable remaining (temp)m4l_pars <- dat %>%
group_by(area_f) %>%
data_grid(temp = mean(temp)) %>%
ungroup() %>%
mutate(temp2 = temp + 3) %>%
pivot_longer(c("temp2", "temp"), values_to = "temp") %>%
dplyr::select(-name) %>%
mutate(temp_sc = (temp - mean(dat$temp)) / sd(dat$temp),
temp_sq_sc = temp_sc*temp_sc) %>%
add_epred_draws(m4l) %>%
rename(linf = .epred) %>%
group_by(temp, area_f) %>%
summarise(linf_median = quantile(linf, prob = 0.5),
linf_lwr = quantile(linf, prob = 0.05),
linf_upr = quantile(linf, prob = 0.95))group_by: one grouping variable (area_f)ungroup: no grouping variablesmutate: new variable 'temp2' (double) with 10 unique values and 0% NApivot_longer: reorganized (temp2) into (name) [was 10x3, now 20x3]mutate: new variable 'temp_sc' (double) with 20 unique values and 0% NA new variable 'temp_sq_sc' (double) with 20 unique values and 0% NArename: renamed one variable (linf)group_by: 2 grouping variables (temp, area_f)summarise: now 20 rows and 5 columns, one group variable remaining (temp)est <- left_join(m4k_pars,
m4l_pars,
by = c("area_f", "temp"))left_join: added 3 columns (linf_median, linf_lwr, linf_upr) > rows only in x 0 > rows only in y ( 0) > matched rows 20 > ==== > rows total 20# Expand by age and calculate length-at-age
est_sum <- est %>%
crossing(age_bc) %>%
mutate(length_mm = linf_median*(1-exp(-k_median*age_bc)),
length_mm_lwr = linf_lwr*(1-exp(-k_lwr*age_bc)),
length_mm_upr = linf_upr*(1-exp(-k_upr*age_bc))) %>%
group_by(area_f) %>%
mutate(temp_cat = ifelse(temp == min(temp), "Mean °C", "Mean + 2°C"))mutate: new variable 'length_mm' (double) with 801 unique values and 0% NA new variable 'length_mm_lwr' (double) with 801 unique values and 0% NA new variable 'length_mm_upr' (double) with 801 unique values and 0% NAgroup_by: one grouping variable (area_f)mutate (grouped): new variable 'temp_cat' (character) with 2 unique values and 0% NApal <- rev(brewer.pal(n = 6, name = "Paired")[c(2, 6)])
est_sum %>%
ggplot(aes(age_bc, length_mm, color = temp_cat)) +
geom_ribbon(aes(age_bc, ymin = length_mm_lwr, ymax = length_mm_upr, fill = temp_cat),
alpha = 0.3, color = NA) +
geom_line() +
coord_cartesian(xlim = c(0, 12)) +
scale_color_manual(values = pal, name = "Temperature") +
scale_fill_manual(values = pal, name = "Temperature") +
facet_wrap(~factor(area_f, rev(order$area)), ncol = 4) +
theme(legend.position = "bottom") +
labs(x = "Age (years)", y = "Predicted length (mm)")
# Difference in size-at-age?
warm <- est_sum %>%
filter(temp_cat == "Mean + 2°C") %>%
dplyr::select(area_f, length_mm, age_bc) %>%
rename(length_mm_warm = length_mm)filter (grouped): removed 410 rows (50%), 410 rows remainingrename: renamed one variable (length_mm_warm)ref <- est_sum %>%
filter(temp_cat == "Mean °C") %>%
dplyr::select(area_f, length_mm, age_bc) %>%
rename(length_mm_ref = length_mm)filter (grouped): removed 410 rows (50%), 410 rows remainingrename: renamed one variable (length_mm_ref)delta <- left_join(warm, ref, by = c("area_f", "age_bc")) %>%
mutate(diff = length_mm_warm - length_mm_ref)left_join: added one column (length_mm_ref) > rows only in x 0 > rows only in y ( 0) > matched rows 410 > ===== > rows total 410mutate (grouped): new variable 'diff' (double) with 401 unique values and 0% NAggplot(delta, aes(age_bc, diff, color = factor(area_f, order$area))) +
geom_line() +
coord_cartesian(xlim = c(0, 12)) +
labs(x = "Age", color = "Area",
y = "Difference in size-at-age with 3 st.dev. increase in temperature (mm)") +
scale_color_manual(values = colors)